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    <title>UTas ePrints - Growth and carcass characteristics of lot-fed Wagyu beef cattle and the estimation of homozygosity from band sharing patterns of random amplied polymorphic DNA markers</title>
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    <meta content="Malau-Aduli, A.E.O." name="eprints.creators_name" />
<meta content="Inoue, S." name="eprints.creators_name" />
<meta content="Richards, T." name="eprints.creators_name" />
<meta content="Howard, A." name="eprints.creators_name" />
<meta content="Thompson, A." name="eprints.creators_name" />
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<meta content="Growth and carcass characteristics of lot-fed Wagyu
beef cattle and the estimation of homozygosity from band sharing
patterns of random amplied polymorphic DNA markers" name="eprints.title" />
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<meta content="RAPD Markers, Wagyu, Carcass Traits" name="eprints.keywords" />
<meta content="The Wagyu breed of beef cattle is renowned for its ability to deposit
high levels of intramuscular fat resulting in highly marbled beef that
meets consumer demands in some niche export markets. We evaluated
the post-weaning growth performance and carcass characteristics
at slaughter of purebred Wagyu steers raised in the feedlot after an
initial backgrounding on grass and silage. Our main aim was to study
the average daily gains (ADG), body condition scores (BCS) and
liveweight (LWT) changes from weaning to slaughter and to estimate
homozygosity and inbreeding coefficients through band sharing
patterns using random amplied polymorphic DNA (RAPD) markers.
LWT, ADG and BCS were monitored monthly from 2005-2006.
Genomic DNA was extracted from blood samples, amplied using
RAPD primers, fragments resolved by gel electrophoresis and banding
patterns elucidated under UV light. A linear increase in liveweight as
age increased was observed and the typical uctuation due to seasonal
variations observed under grazing conditions was unnoticeable.
Average LWT ranged from an initial 110kg to 660kg, ADG ranged
from 0.7–2.0kg/day and BCS reached the maximum of 5 at the end
of the experiment. Average LWT at slaughter was 574kg with a hot
carcass weight of 329kg and a dressing percentage of 57%. Mean eye
muscle area was 94cm2, eye muscle width 8cm and eye muscle length
16cm. Marbling score was 3, subcutaneous fat depth of 17cm and
total trimmed fat weight was 34kg. Average saleable meat yield
based on the 4 most valuable hind muscles were: Round (11.7kg),
Topside (19.3kg), Rump (13.6kg) and Silverside (15.8kg). Average
band sharing frequencies ranged from 0.60 to 0.96 with estimated
inbreeding coefcients ranging from 0.5% to 7%, respectively. It was
concluded that the inbreeding level was low, negligible and not in
any way detrimental to meat yield and carcass quality destined for the
Japanese market. Finally, RAPD markers were not versatile enough toclearly differentiate between the fastest and slowest growing animals
within the Wagyu breed.
" name="eprints.abstract" />
<meta content="2007-07-08" name="eprints.date" />
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<meta content="Malau-Aduli, A.E.O. and Inoue, S. and Richards, T. and Howard, A. and Thompson, A. (2007) Growth and carcass characteristics of lot-fed Wagyu beef cattle and the estimation of homozygosity from band sharing patterns of random amplied polymorphic DNA markers. Journal of Animal Science, 85 (Suppl 1). pp. 189-190. ISSN 1525-3163" name="eprints.citation" />
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beef cattle and the estimation of homozygosity from band sharing
patterns of random amplied polymorphic DNA markers" name="DC.title" />
<meta content="Malau-Aduli, A.E.O." name="DC.creator" />
<meta content="Inoue, S." name="DC.creator" />
<meta content="Richards, T." name="DC.creator" />
<meta content="Howard, A." name="DC.creator" />
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<meta content="The Wagyu breed of beef cattle is renowned for its ability to deposit
high levels of intramuscular fat resulting in highly marbled beef that
meets consumer demands in some niche export markets. We evaluated
the post-weaning growth performance and carcass characteristics
at slaughter of purebred Wagyu steers raised in the feedlot after an
initial backgrounding on grass and silage. Our main aim was to study
the average daily gains (ADG), body condition scores (BCS) and
liveweight (LWT) changes from weaning to slaughter and to estimate
homozygosity and inbreeding coefficients through band sharing
patterns using random amplied polymorphic DNA (RAPD) markers.
LWT, ADG and BCS were monitored monthly from 2005-2006.
Genomic DNA was extracted from blood samples, amplied using
RAPD primers, fragments resolved by gel electrophoresis and banding
patterns elucidated under UV light. A linear increase in liveweight as
age increased was observed and the typical uctuation due to seasonal
variations observed under grazing conditions was unnoticeable.
Average LWT ranged from an initial 110kg to 660kg, ADG ranged
from 0.7–2.0kg/day and BCS reached the maximum of 5 at the end
of the experiment. Average LWT at slaughter was 574kg with a hot
carcass weight of 329kg and a dressing percentage of 57%. Mean eye
muscle area was 94cm2, eye muscle width 8cm and eye muscle length
16cm. Marbling score was 3, subcutaneous fat depth of 17cm and
total trimmed fat weight was 34kg. Average saleable meat yield
based on the 4 most valuable hind muscles were: Round (11.7kg),
Topside (19.3kg), Rump (13.6kg) and Silverside (15.8kg). Average
band sharing frequencies ranged from 0.60 to 0.96 with estimated
inbreeding coefcients ranging from 0.5% to 7%, respectively. It was
concluded that the inbreeding level was low, negligible and not in
any way detrimental to meat yield and carcass quality destined for the
Japanese market. Finally, RAPD markers were not versatile enough toclearly differentiate between the fastest and slowest growing animals
within the Wagyu breed.
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    <h1 class="ep_tm_pagetitle">Growth and carcass characteristics of lot-fed Wagyu beef cattle and the estimation of homozygosity from band sharing patterns of random amplied polymorphic DNA markers</h1>
    <p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Malau-Aduli, A.E.O.</span> and <span class="person_name">Inoue, S.</span> and <span class="person_name">Richards, T.</span> and <span class="person_name">Howard, A.</span> and <span class="person_name">Thompson, A.</span> (2007) <xhtml:em>Growth and carcass characteristics of lot-fed Wagyu beef cattle and the estimation of homozygosity from band sharing patterns of random amplied polymorphic DNA markers.</xhtml:em> Journal of Animal Science, 85 (Suppl 1). pp. 189-190. ISSN 1525-3163</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a onmouseover="EPJS_ShowPreview( event, 'doc_preview_3711' );" onmouseout="EPJS_HidePreview( event, 'doc_preview_3711' );" href="http://eprints.utas.edu.au/2803/1/Malau-Aduli_et_al_2007_JAnimSci85Suppl1Wagyu_RAPD.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" border="0" class="ep_doc_icon" /></a><div class="ep_preview" id="doc_preview_3711"><table><tr><td><img alt="" src="http://eprints.utas.edu.au/2803/thumbnails/1/preview.png" border="0" class="ep_preview_image" /><div class="ep_preview_title">Preview</div></td></tr></table></div></td><td valign="top"><a href="http://eprints.utas.edu.au/2803/1/Malau-Aduli_et_al_2007_JAnimSci85Suppl1Wagyu_RAPD.pdf"><span class="ep_document_citation">PDF</span></a> - Requires a PDF viewer<br />499Kb</td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://jas.fass.org/">http://jas.fass.org/</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">The Wagyu breed of beef cattle is renowned for its ability to deposit&#13;
high levels of intramuscular fat resulting in highly marbled beef that&#13;
meets consumer demands in some niche export markets. We evaluated&#13;
the post-weaning growth performance and carcass characteristics&#13;
at slaughter of purebred Wagyu steers raised in the feedlot after an&#13;
initial backgrounding on grass and silage. Our main aim was to study&#13;
the average daily gains (ADG), body condition scores (BCS) and&#13;
liveweight (LWT) changes from weaning to slaughter and to estimate&#13;
homozygosity and inbreeding coefficients through band sharing&#13;
patterns using random amplied polymorphic DNA (RAPD) markers.&#13;
LWT, ADG and BCS were monitored monthly from 2005-2006.&#13;
Genomic DNA was extracted from blood samples, amplied using&#13;
RAPD primers, fragments resolved by gel electrophoresis and banding&#13;
patterns elucidated under UV light. A linear increase in liveweight as&#13;
age increased was observed and the typical uctuation due to seasonal&#13;
variations observed under grazing conditions was unnoticeable.&#13;
Average LWT ranged from an initial 110kg to 660kg, ADG ranged&#13;
from 0.7–2.0kg/day and BCS reached the maximum of 5 at the end&#13;
of the experiment. Average LWT at slaughter was 574kg with a hot&#13;
carcass weight of 329kg and a dressing percentage of 57%. Mean eye&#13;
muscle area was 94cm2, eye muscle width 8cm and eye muscle length&#13;
16cm. Marbling score was 3, subcutaneous fat depth of 17cm and&#13;
total trimmed fat weight was 34kg. Average saleable meat yield&#13;
based on the 4 most valuable hind muscles were: Round (11.7kg),&#13;
Topside (19.3kg), Rump (13.6kg) and Silverside (15.8kg). Average&#13;
band sharing frequencies ranged from 0.60 to 0.96 with estimated&#13;
inbreeding coefcients ranging from 0.5% to 7%, respectively. It was&#13;
concluded that the inbreeding level was low, negligible and not in&#13;
any way detrimental to meat yield and carcass quality destined for the&#13;
Japanese market. Finally, RAPD markers were not versatile enough toclearly differentiate between the fastest and slowest growing animals&#13;
within the Wagyu breed.&#13;
</p></div><table style="margin-bottom: 1em" border="0" cellpadding="3" class="not_ep_block"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Keywords:</th><td valign="top" class="ep_row">RAPD Markers, Wagyu, Carcass Traits</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/270207.html">270000 Biological Sciences &gt; 270200 Genetics &gt; 270207 Quantitative Genetics</a><br /><a href="http://eprints.utas.edu.au/view/subjects/300401.html">300000 Agricultural, Veterinary and Environmental Sciences &gt; 300400 Animal Production &gt; 300401 Animal Breeding</a><br /><a href="http://eprints.utas.edu.au/view/subjects/300406.html">300000 Agricultural, Veterinary and Environmental Sciences &gt; 300400 Animal Production &gt; 300406 Animal Growth and Development</a><br /><a href="http://eprints.utas.edu.au/view/subjects/270800.html">270000 Biological Sciences &gt; 270800 Biotechnology</a></td></tr><tr><th valign="top" class="ep_row">Collections:</th><td valign="top" class="ep_row">UNSPECIFIED</td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">2803</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Dr Aduli E.O. Malau-Aduli</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">04 Jan 2008 11:59</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">07 Feb 2008 09:30</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=2803;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&amp;eprintid=2803">item control page</a></p>
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